Enzymes Lab

Lab: Enzymes Experiment 1: Effect of pH on Enzyme military action Date Objective: To study the forcefulness of pH on enzymatic exercise. Material Required: 1. Catalase enzyme 2. irrigate system2 3. Scaled Beaker 4. Dropper 5. Acidic result 6. Basic solution theory Does change in pH affects an enzymatic act? scrutiny of Hypothesis Enzyme may stick tabu stirred by various factors. pH is also one out of these? It could be analyzed via introduction of change in pH specialize via addition of contrary acidulous and base in reaction mixture. For instance, in the reaction mixture of catalase with its substrate such as; H2O2. The reaction of Catalase enzyme on its substrate might be represented as follows: H2O2=H2O+1/2O2 If pH leave affect its activity thusly due to change in O2 production the take of spume would also change. information hookup Data might be collected via measurement of the level of foam in the scaled beaker. turn off 1: Effect of pH on Enzyme activity Tube| pH| Level of foam (in mm.)| | | 1| 2| 4| | | 2| 14| 1| | | 3| 8| 102| | | Data abridgment Data analysis may be done via conclusion a relation between changed pH hold in and level of O2 produced in this way. Result According to the data, there is reduction in activity of enzyme in acidic (pH = 2) and highly basic condition (pH = 14) due to slight foam (means less O2) production.

But, at somewhat basic condition (pH=8) the activity of enzyme is highest. Graphical Representation Enzyme activity (y axis) depends on valuate of pH (x-axi s) hence, there might be spy a flower in g! raph where the value of enzymatic activity is maximum. O 8 Discussion The feign of pH on enzymatic activity might be due to change in the ionisation of amino acid residues and disruption in the non-covalent interactions. Conclusion Hence; it could be observed that our hypothesis that pH might also...If you want to get a full essay, order it on our website: OrderCustomPaper.com

If you want to get a full essay, visit our page: write my paper